植物轉化技術為基礎科學和轉化研究提供了大量的機會。幾種農桿菌介導的植物轉化方法可用于水稻愈傷組織的誘導和再生。經常使用的轉化程序需要時間和熟練的勞動力，并且受到組織再生能力的限制。本文以pCAMBIA-amiR820為模型構建，描述了一種簡單、健壯、與組織培養無關的水稻種子轉化方法。從轉化種子中獲得的植株在抗生素培養基上進行篩選，并通過分子技術進行轉基因整合和表達檢測。由此產生的轉基因幼苗包括穩定的轉化體和嵌合體的混合物；然而,通過該方法第一代種子含有穩定整合的轉基因。
PMID: 33471324 DOI: 10.1007/978-1-0716-1068-8_4
Plant transformation technology offers ample opportunities for basic scientific and translational research. Several Agrobacterium-mediated plant transformation protocols are available, for transforming rice, through callus initiation and regeneration. The regularly used transformation procedures require time and skilled labor and are limited by the regeneration capabilities of the tissue. Here we describe a simple, robust and tissue culture-independent method for transformation of rice seeds using pCAMBIA-amiR820 as model construct. Plants obtained from the transformed seeds were selected on antibiotic media and tested for transgene integration and expression by molecular techniques. The transgenic seedlings thus produced include a mix of stable transformants and chimeras; however the first generation seeds contained stably integrated transgene.
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